abstract：:Cultured rat cerebellar astrocytes, loaded with the Ca2+-sensitive fluorescent dyes Fura-2 or Fluo-3, responded with cytoplasmic Ca2+ transients, when the external K+ concentration was reduced from 5 mM to below 1 mM. Ca2+ transients were generated after changing to a saline containing 0.2 mM K+ in 82% of the cells (n...

journal_title：Cell calcium

authors： Dallwig R,Vitten H,Deitmer JW

更新日期：2000-10-01 00:00:00

abstract：:Calcium and calcium-dependent systems have been long implicated in the induction of epilepsy. We have previously observed that intracellular calcium ([Ca2+]i) levels remain elevated in cells undergoing epileptogenesis in the hippocampal neuronal culture (HNC) model. In this study, we employed the hippocampal neuronal ...

journal_title：Cell calcium

authors： Pal S,Limbrick DD Jr,Rafiq A,DeLorenzo RJ

更新日期：2000-09-01 00:00:00

abstract：:A whole range of cell functions are regulated by the free cytosolic Ca(2+)concentration. Activator Ca(2+)from the extracellular space enters the cell through various types of Ca(2+)channels and sometimes the Na(+)/Ca(2+)-exchanger, and is actively extruded from the cell by Ca(2+)pumps and Na(+)/Ca(2+)-exchangers. Acti...

journal_title：Cell calcium

authors： Missiaen L,Robberecht W,van den Bosch L,Callewaert G,Parys JB,Wuytack F,Raeymaekers L,Nilius B,Eggermont J,De Smedt H

更新日期：2000-07-01 00:00:00

abstract：:In human embryonic kidney (HEK) cells stably transfected with green fluorescent protein targeted to the endoplasmic reticulum (ER), elevation of intracellular Ca2+ ([Ca2+]i) altered ER morphology, making it appear punctate. Electron microscopy revealed that these punctate structures represented circular and branched r...

journal_title：Cell calcium

authors： Ribeiro CM,McKay RR,Hosoki E,Bird GS,Putney JW Jr

更新日期：2000-03-01 00:00:00

abstract：:The concentration of free Ca2+ in the cytoplasm and organelles of individual mouse pancreatic beta-cells was estimated with dual wavelength microfluorometry and the indicators Fura-2 and furaptra. Measuring the increase of cytoplasmic Ca2+ resulting from intracellular mobilization of the ion in ob/ob mouse beta-cells,...

journal_title：Cell calcium

authors： Tengholm A,Hellman B,Gylfe E

更新日期：2000-01-01 00:00:00

abstract：:MAP kinases are a family of protein kinases that are ubiquitously expressed and play roles in most signal transduction pathways. They are activated within protein kinase cascades consisting of at least three kinases acting in series. In many, if not all cases, the three-kinase cascade, conveniently referred to as a MA...

journal_title：Cell calcium

authors： Karandikar M,Cobb MH

更新日期：1999-11-01 00:00:00

abstract：:In mammals Ca2+ concentration in the extracellular fluids ([Ca2+]o) is essential for a number of vital processes varying from bone mineralization to blood coagulation, regulation of enzymatic processes, modulation of permeability and excitability of plasma membranes. For this reason [Ca2+]o is under strict control of ...

journal_title：Cell calcium

authors： Riccardi D

更新日期：1999-09-01 00:00:00

abstract：:Intracytoplasmic sperm injection (ICSI) into mammalian eggs induces repetitive rises in intracellular Ca2+ concentration ([Ca2+]i) which are the pivotal signal in fertilization. Spatiotemporal aspects of [Ca2+]i rises following ICSI into the periphery of mouse eggs were investigated with high-speed confocal microscopy...

journal_title：Cell calcium

authors： Sato MS,Yoshitomo M,Mohri T,Miyazaki S

更新日期：1999-07-01 00:00:00

abstract：:Membrane deformation induced by a mechanical stimulus increases the [Ca2+]i in cultured retinal pigment epithelial (RPE) cells, and in many other cell types. In this study, confocal microscopy and Ca(2+)-measurements using the fluorescent dye fluo-3 were used to measure the spatiotemporal characteristics of the Ca(2+)...

journal_title：Cell calcium

authors： Stalmans P,Himpens B

更新日期：1999-06-01 00:00:00

abstract：:The adenine nucleotide translocator (ANT) is an autoantigen in myocarditis and dilated cardiomyopathy. Carrier-specific antibodies impair myocardial energy metabolism and heart function. They cross-react with a myolemmal calcium channel and alter calcium fluxes in isolated myocytes. To test whether antibodies against ...

journal_title：Cell calcium

authors： Schulze K,Heineman FW,Schultheiss HP,Balaban RS

更新日期：1999-05-01 00:00:00

abstract：:Cardiac muscle excitation-contraction coupling is controlled by the Ca(2+)-induced Ca2+ release mechanism. The present study examines the effects of a calmodulin antagonist W-7 on Ca2+ current (ICa)-induced Ca2+ release in whole cell-clamped rat ventricular myocytes. Exposure of cells to W-7 suppressed ICa, but the in...

journal_title：Cell calcium

authors： Suzuki YJ,Wang W,Morad M

更新日期：1999-03-01 00:00:00

abstract：:Fura-2 and BAPTA were previously shown to be competitive antagonists of inositol trisphosphate (InsP3) receptors, but for practical reasons the analyses were performed at pH 8.3. We recently developed a scintillation proximity assay (SPA) for pure cerebellar InsP3 receptors which allows low affinity interactions to be...

journal_title：Cell calcium

authors： Morris SA,Correa V,Cardy TJ,O'Beirne G,Taylor CW

更新日期：1999-02-01 00:00:00

abstract：:The role of intracellular calcium stores in stimulus-secretion coupling in the pancreatic beta-cell is largely unknown. We report here that tetracaine stimulates insulin secretion from collagenase-isolated mouse islets of Langerhans in the absence of glucose or extracellular calcium. We also found that the anesthetic ...

journal_title：Cell calcium

authors： Mears D,Leighton X,Atwater I,Rojas E

更新日期：1999-01-01 00:00:00

abstract：:A procedure for calibration of fluorescence signals from adult rat heart cells loaded with the -AM ester of fura-2 is described. Calibration is complicated by dye compartmentation and potentially incomplete dye hydrolysis. These problems were overcome by subtracting from fluorescence transients the non-cytosolic (mito...

journal_title：Cell calcium

authors： Haworth RA,Redon D

更新日期：1998-10-01 00:00:00

abstract：:Light-sensitive channels encoded by the Drosophila transient receptor potential-like gene (trpl) are activated in situ by an unknown mechanism requiring activation of Gq and phospholipase C (PLC). Recent studies have variously concluded that heterologously expressed TRPL channels are activated by direct Gq-protein int...

journal_title：Cell calcium

authors： Hardie RC,Raghu P

更新日期：1998-09-01 00:00:00

abstract：:Here we report on the feasibility of using replication deficient adenoviruses to modify signal transduction systems in epithelia. We constructed two viruses, one expressing a dominant negative mutant of the alpha-subunit of Gq (Ad-EF1-dnG alpha q) and the other expressing the wild-type alpha-subunit of Gq (Ad-EF1-wtG ...

journal_title：Cell calcium

authors： Poronnik P,O'Mullane LM,Harding EA,Greger R,Cook DI

更新日期：1998-08-01 00:00:00

abstract：:We tested a mixture of Calcium-Green-1 (CG-1) and Brilliantsulfaflavine (BS) for dual excitation ratiometric measurements of the intracellular free calcium concentration ([Ca2+]i) in bovine adrenal chromaffin cells. Dyes were coloaded (without being molecularly linked to each other) in the whole-cell configuration of ...

journal_title：Cell calcium

authors： Oheim M,Naraghi M,Müller TH,Neher E

更新日期：1998-07-01 00:00:00

abstract：:The sphingosine derivatives sphingosylphosphorylcholine (SPC) and sphingosine-1-phosphate (S1P) caused a similar elevation of the intracellular Ca2+ concentration ([Ca2+]i) in an immortalized airway epithelial cell line (CFNP9o-) incubated in Ca(2+)-free medium. The maximal effect was obtained with 2 microM SPC and 0....

journal_title：Cell calcium

authors： Orlati S,Porcelli AM,Hrelia S,Rugolo M

更新日期：1998-06-01 00:00:00

abstract：:Near-membrane [Ca2+] may differ significantly from bulk cytosolic [Ca2+], particularly during rapid Ca2+ signalling events related to cardiac muscle excitation-contraction coupling. We used the lipophilic membrane-associated Ca2+ indicator Ca(2+)-Green C-18 (C-18) and laser-scanning confocal microscopy to detect extra...

journal_title：Cell calcium

authors： Blatter LA,Niggli E

更新日期：1998-05-01 00:00:00

abstract：:Synaptic transmission is a key signaling event, whereby an action potential-induced release of chemical neurotransmitters again generates a positive or negative electrical activity via opening of postsynaptic channels. Thereafter, information spreads through space, from the postsynaptic membranes to the dendrites, to ...

journal_title：Cell calcium

authors： Bito H

更新日期：1998-02-01 00:00:00

abstract：:Ca2+ influx into secretory cells of the intact salivary gland of the blowfly Calliphora erythrocephala elicited by the agonist 5-hydroxytryptamine (5-HT) or the Ca2+ uptake inhibitor thapsigargin was studied by using Fura-2 and digital fluorescence imaging and by recordings of the transepithelial potential. Applicatio...

journal_title：Cell calcium

authors： Zimmermann B

更新日期：1998-01-01 00:00:00

abstract：:Several proteins have been identified that associate with calcium release channels and potentially regulate their function. Using tacrolimus as a pharmacological tool, we investigated whether the immunophilin FKBP12 modulates ryanodine receptor channels in intestinal smooth muscle. Results with PCR demonstrated the pr...

journal_title：Cell calcium

authors： Bielefeldt K,Sharma RV,Whiteis C,Yedidag E,Abboud FM

更新日期：1997-12-01 00:00:00

abstract：:There is a close association of peripheral sarcoplasmic reticulum (SR), containing IP3 receptors, and regions of the plasma membrane enriched in the Na+/Ca2+ exchanger in smooth muscle. We have tested the possibility in rat uterine smooth muscle that Ca2+ released from the SR is preferentially removed from the cytosol...

journal_title：Cell calcium

authors： Taggart MJ,Wray S

更新日期：1997-11-01 00:00:00

abstract：:The actions of the novel calcium (Ca2+) channel antagonist mibefradil (Ro 40-5967), a selective T-type channel blocker in myocardium, were investigated in embryonic rat spinal motoneurones maintained in culture. Whole-cell currents were recorded with the patch-clamp technique. Motoneurones displayed transient, low-vol...

journal_title：Cell calcium

authors： Viana F,Van den Bosch L,Missiaen L,Vandenberghe W,Droogmans G,Nilius B,Robberecht W

更新日期：1997-10-01 00:00:00

abstract：:The correlation between the intracellular Ca2+ concentration ([Ca2+]i) and membrane potential in monolayers of density-arrested normal rat kidney (NRK) fibroblasts was investigated. Using the fluorescent probe Fura-2, spontaneous repetitive spike-like increases in [Ca2+]i (Ca2+ spikes) were observed that were synchron...

journal_title：Cell calcium

authors： de Roos AD,Willems PH,Peters PH,van Zoelen EJ,Theuvenet AP

更新日期：1997-09-01 00:00:00

abstract：:In previous studies we have characterised various properties of capacitative Ca2+ entry (CCE) in different epithelia. After Ca2+ store depletion with PLC/InsP3-coupled agonists or by inhibition of store Ca2+ uptake, with for example thapsigargin, Ca2+ influx is activated. This leads to a sustained cellular response (e...

journal_title：Cell calcium

authors： Gordjani N,Nitschke R,Greger R,Leipziger J

更新日期：1997-08-01 00:00:00

abstract：:Maitotoxin (MTX), a water-soluble polyether obtained from the marine dinoflagellate Gambierdiscus toxicus increased intracellular calcium in a concentration-dependent manner in fibroblasts obtained from human skin. The effect of this toxin was both saturable and of high affinity, showing an apparent half activation co...

journal_title：Cell calcium

authors： Gutierrez D,Díaz de León L,Vaca L

更新日期：1997-07-01 00:00:00

abstract：:We describe the construction of a high-resolution confocal laser-scanning microscope, and illustrate its use for studying elementary Ca2+ signalling events in cells. An avalanche photodiode module and simple optical path provide a high efficiency system for detection of fluorescence signals, allowing use of a small co...

journal_title：Cell calcium

authors： Parker I,Callamaras N,Wier WG

更新日期：1997-06-01 00:00:00

abstract：:The effects of the unsaturated fatty acids, arachidonic and oleic acid, on the influx of Ca2+ activated by depletion of intracellular stores with thapsigargin were investigated in various cell types. By using a Ca2+ free/Ca2+ reintroduction protocol, we observed that arachidonic acid (2 to 5 microM) inhibited thapsiga...

journal_title：Cell calcium

authors： Gamberucci A,Fulceri R,Benedetti A

更新日期：1997-05-01 00:00:00

abstract：:Inhibition by Mg2+ ions of exocytotic secretion from permeabilised eosinophils, stimulated by Ca2+ and GTP gamma S, and in the presence and absence of ATP, has been examined. While Mg2+ inhibits release of aryl sulphatase, hexosaminidase and peroxidase, we found no evidence that this occurs by competition at a Ca(2+)-...

journal_title：Cell calcium

authors： Larbi KY,Gomperts BD

更新日期：1997-03-01 00:00:00

abstract：:Using whole cell patch clamp recordings on unfertilized eggs of the ascidian Ciona intestinalis, we are able to detect ryanodine receptors within the oocytes. Our approach is based on measurements of the voltage-activated inward calcium currents. Two types of Ca2+ currents have been described on the oocyte membrane of...

journal_title：Cell calcium

authors： Arnoult C,Albrieux M,Antoine AF,Grunwald D,Marty I,Villaz M

更新日期：1997-02-01 00:00:00

abstract：:Cultured human skeletal muscle cells are frequently used as a model to study muscle pathology, in which Ca2+ homeostasis might be affected. However, their excitation-contraction (E-C) coupling has been poorly investigated. In order to elucidate E-C coupling of cultured muscle cells, we activated the acetylcholine rece...

journal_title：Cell calcium

authors： Benders AA,Oosterhof A,Wevers RA,Veerkamp JH

更新日期：1997-01-01 00:00:00

abstract：:Here, we report the characterization of a human cDNA coding for the recently published amino acid sequence of a calcium-binding S100 protein, S100A12 (CGRP, calgranulin C, CAAF1, p6). The exon/intron structure of the S100A12 gene is similar to most other S100 genes. It is composed of three exons which are divided by t...

journal_title：Cell calcium

authors： Wicki R,Marenholz I,Mischke D,Schäfer BW,Heizmann CW

更新日期：1996-12-01 00:00:00

abstract：:Many fluorescent Ca indicators, particularly those loaded as acetoxymethyl (AM)-ester derivatives, are known to become compartmentalized into organelles. This property can be exploited to measure changes in free [Ca] in subcellular compartments, including the inositol (1,4,5)-trisphosphate-sensitive store. However, qu...

journal_title：Cell calcium

authors： Hofer AM,Schulz I

更新日期：1996-09-01 00:00:00

abstract：:The use of high resolution imaging techniques has revealed new forms of dendritic signal integration in neurones. In contrast to electrical signals that have a more widespread influence on the cell, brief Ca2+ transients resulting from synaptic activation are often restricted to a small part of the dendritic tree. In ...

journal_title：Cell calcium

authors： Eilers J,Plant T,Konnerth A

更新日期：1996-08-01 00:00:00

abstract：:The effect of the uterotonic pituitary hormone oxytocin on the regulation of intracellular calcium concentration ([Ca2+]i) was studied in single cells of a smooth muscle cell line derived from human non-pregnant myometrium. [Ca2+]i was measured with fluorescence microscopy, and by recording the activity of Ca(2+)-acti...

journal_title：Cell calcium

authors： Holda JR,Oberti C,Perez-Reyes E,Blatter LA

更新日期：1996-07-01 00:00:00

abstract：:We investigated whether the expression of human alpha-parvalbumin affects depolarization-induced elevations of the cytosolic free calcium concentration ([Ca2+]i) in human neuroblastoma SKNBE2 cells. A full length human parvalbumin cDNA was cloned by PCR from human cerebellum and transiently transfected into SKNBE2 cel...

journal_title：Cell calcium

authors： Dreessen J,Lutum C,Schäfer BW,Heizmann CW,Knöpfel T

更新日期：1996-06-01 00:00:00

abstract：:Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...

journal_title：Cell calcium

authors： Harrington MA,Thompson SH

更新日期：1996-05-01 00:00:00

abstract：:Human CD3-, CD16+ natural killer cells (NKs) induced necrosis in the cell line K562 and the minor population of MOLT-4, while induced apoptosis in the majority of MOLT-4, in one-to-one cell interaction. Simultaneous Ca2+ imaging of NK and target cells revealed remarkable differences in changes of cytosolic Ca2+ concen...

journal_title：Cell calcium

authors： Honda Y,Miyazaki S

更新日期：1996-04-01 00:00:00

abstract：:Agonist-induced calcium (Ca) mobilization is accompanied by Ca efflux, presumably reflecting the rise in Ca concentration at the cytosolic surface of the cell membrane. We studied the relationship between Ca efflux and intracellular Ca mobilization in Xenopus oocytes. Elevation of cytosolic Ca by a direct injection of...

journal_title：Cell calcium

authors： Shapira H,Lupu-Meiri M,Lipinsky D,Oron Y

更新日期：1996-03-01 00:00:00